They providing a comprehensive approach to ensure the safety and quality of cosmetic products. The workflow of microbiological testing for cosmetics according to this standard includes the following steps.
1- Microbiology Sampling
Raw materials and finished products must be sampled for microbiological testing, with a sampling plan based on the microbiological risk of the product. The analysis approach and number of samples collected must be determined based on the product's intended use, target market, and manufacturing process. The microbial limits for cosmetic products must be defined, including limits for bacterial counts, total viable count, and mould counts, among others. The microbiological limits must be based on safety considerations and product efficacy, and they must comply with regulatory requirements.
2- Microbiological Testing
Total Viable Count
The total viable count of microorganisms in the cosmetic product is determined by conducting an aerobic plate count of the sample. The sample is incubated for a specific period, and the number of colonies is counted.
Bacterial Counts
The bacterial count of specific microorganisms, such as Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa, are determined using selective media and incubation conditions specific to the microorganism.
Mould Count
The mould count of microorganisms in the cosmetic product is determined using selective media and incubation conditions specific to the mould.
3- Quality Control
Quality control testing must be conducted to ensure the accuracy and reliability of the microbiological testing results. Quality control testing includes conducting positive and negative control tests, assessing the media and incubation conditions, and conducting repeat testing of the same sample.
In conclusion, microbiological testing of cosmetics is an essential aspect of ensuring the safety and quality of cosmetic products. ISO 18415:2017 provides guidelines for conducting microbiological testing of cosmetic products, including a comprehensive approach to sampling, microbial limits, and analysis. The workflow of microbiological testing includes sampling, determination of microbial limits, and specific testing for bacterial counts, total viable count, mould counts, and quality control testing to ensure the accuracy of results.
